We are seeking a highly motivated and creative scientist for a research technician position in the laboratory of Michael Eck, M.D., Ph.D. at the Dana-Farber Cancer Institute and Harvard Medical School (ecklab.dana-farber.org). The Eck lab uses biochemical and structural methods to study kinases that are important in cancer. We are especially interested in understanding molecular mechanisms of kinase regulation, oncogenic activation, and drug resistance with the goal of developing novel cancer therapies. Technicians work closely with a postdoc or scientist in the lab, are included as an author on relevant publications, and often pursue graduate or medical school. Technicians are also invited to attend a variety of departmental seminars and events.

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Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow’s physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

Under general supervision, the research technician will perform a variety of laboratory and investigative procedures including routine experimental techniques such as cloning, Western blot, protein purification, biochemical assays, and tissue culture. Technicians will need to collect, verify, record, and compile data and information in support of research.

• Perform various molecular biology techniques such as PCR, Gibson assembly, DNA sequencing, agarose gel electrophoresis, bacterial transformation, and DNA isolation.
• Protein expression in different expression systems, optimization of expression level, and purification methods via FPLC.
• Protein analysis with SDS-PAGE, Coomassie staining, and Western blotting.
• Conduct standardized biological, pharmacological, or biochemical assays.
• Perform various investigative procedures and operate a variety of instruments and other scientific equipment.
• Set up, adjust, calibrate, clean, maintain, and troubleshoot equipment.
• Perform cell and tissue culture following BSL-2 safety protocols.
• Analyze experimental data and interpret results to write reports and summaries of findings.
• Keep an accurate record of all work.
• Present research updates during lab meetings.
• Read and interpret literature applicable to research areas as required.

One full-time position will be filled. We are looking for candidates who have at least one year of availability.

• Bachelor's Degree required with a major in biology, chemistry, or a related discipline. Degree may be in progress at the time of application but must be completed prior to beginning work.
• Laboratory research experience in biochemistry, molecular biology, cell biology, or a related area is highly desirable.
• Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.
• Ability to analyze information and use logic to address work-related issues and problems.
• Must be able to follow standard operating procedures to ensure the safety of all members of the lab and quality of experimental results.
• Must have attention to detail and be thorough in completing work tasks.
• Ability to work within a team environmentÂ

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

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At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong. As relentless as we are in our mission to reduce the burden of cancer for all, we are equally committed to diversifying our faculty and staff. Cancer knows no boundaries and when it comes to hiring the most dedicated and diverse professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.

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